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. 2019 Dec 23;177(1):145–160. doi: 10.1111/bph.14853

Figure 6.

Figure 6

Nox4, at least partly, mediates the down‐regulation of fibronectin expression by Cx32 in HG‐induced GMCs. (a and b) The expression of Nox4 in the glomeruli was shown by immumohistochemical staining (400× magnification). (c) Cx32 overexpression decreased the expression of Nox4. # P < .05, significantly different from NG; * P < .05, significantly different from HG. (d) Cx32 knockdown increased Nox4 expression. # P < .05, significantly different from NG; * P < .05, significantly different from HG. (e) Cx32 co‐localized with Nox4 in GMCs under normal conditions, as revealed by immunofluorescence assay. Red fluorescence indicates the localization of Cx32. Green fluorescence indicates Nox4. Blue fluorescence indicates nuclei. (f) Immunoprecipitation analysis was used to detect the interaction between Cx32 and Nox4 in GMCs. (g) Overexpression of Nox4 under HG conditions inhibited the down‐regulation effects of Cx32 in the expression of fibronectin . # P < .05, significantly different from NG; * P < .05, significantly different from HG; $ P < .05, significantly different from HG + Cx32. The data are presented as the means ± SD; n = 5.