Skip to main content
. 2019 Nov 28;118(2):518–531. doi: 10.1016/j.bpj.2019.11.3385

Figure 2.

Figure 2

ATPase activity of co-reconstituted proteoliposomes containing SERCA and SLN. (A) ATPase activity of SERCA was reconstituted in the absence (SERCA; circles) and in the presence of wild-type SLN (SERCA + SLN; triangles) and Asn4-Ala SLN (SERCA + N4A; squares) at a 1:5 molar ratio. The calcium affinity values (KCa) are shown in the inset table, and all curves have been normalized to the maximal activity (V/Vmax). Notice the gain-of-function behavior observed for the Asn4-Ala mutant (larger rightward shift of the ATPase activity curve). (B) ATPase activity of SERCA reconstituted in the absence of SLN (black circles) and in the presence of 1:2 molar ratio (white circles) and a 1:5 molar ratio (triangles) of SERCA to wild-type SLN. The calcium affinity (KCa) and maximal activity (Vmax) values are shown in the inset table. Notice the decrease in the Vmax of SERCA at the 1:5 molar ratio of SERCA-SLN. Each data point is the mean ± standard error (n ≥ 3).