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. 2019 Dec 27;32:160–167. doi: 10.1016/j.molmet.2019.12.012

Figure 2.

Figure 2

Effect of GAS5 knockdown in beta cells and rescue by GAS5 HREM. A. GAS5 expression is reduced to ≈40% after transfecting cells with LNA gapmeR against GAS5. B. GSIS assay upon GAS5 knockdown in EndoC-βH1 cells. C. Measurement of apoptosis upon GAS5 knockdown. D. GSIS measurement upon GAS5 KD in combination with dexamethasone treatment in EndoC-βH1 with or without RU486. E. Rescue of GSIS by GAS5 hormone response element mimic (HREM) in GC-treated cells. The stem-loop (SL) sequence from GAS5 is non-functional and is used as a negative control. For all the experiments, the data are presented as an average of n = 4–16 biological replicates, mean ± SEM, §, *p < 0.05 and §§, **p < 0.01.