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. 2020 Jan 20;30:101430. doi: 10.1016/j.redox.2020.101430

Fig. 3.

Fig. 3

legend: A-B) GRP78 and NF-κB1 mRNA levels. ARPE-19 cells were treated with 1 μl/ml dimethyl sulfoxide (DMSO); 0.5 mM taurohyodeoxycholic acid (TUDCA); 10 μg/ml tunicamycin (TM) and 10 μg/ml TM + 0.5 mM TUDCA for 24 h. Fold change (2−ΔΔCt) in mRNA levels of NFκB1 and GRP78. Values are mean ± SD, n = 3. Statistical analysis was performed by one way analysis of variance with all pairwise multiple comparison procedures done by Holm-Sidak method. *, p < 0.05 vs. DMSO, TUDCA and TM + TUDCA. #, p < 0.05 vs. DMSO and TUDCA. C-D) Representative Immunofluorescent staining of GRP78 and NFκB in ARPE-19 cells, respectively. E-F) Quantitation of fluorescence staining was estimated by ImageJ software. Data shown are representative of 3 separate experiments and values are given as mean ± SD. Statistical analysis was performed by One Way Analysis of Variance and all pairwise multiple comparison procedures done by Tukey test. *, p < 0,001 vs. Control, DMSO, TUDCA and TM + TUDCA. #, p < 0,001 vs. Control, DMSO, TUDCA.