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. 2019 Nov 7;10(1):159–170. doi: 10.1016/j.apsb.2019.11.001

Figure 3.

Figure 3

Both SLC7A5/LAT1 and SLC2A1/GLUT1 are the direct targets of miR-328-3p. (A) Computational analyses identified multiple putative MREs for miR-328-3p within the 3′UTR of both SLC7A5 and SLC2A1 transcripts. (B) High levels of miR-328-3p were generated from hBERA/miR-328 in HEK293 cells, as determined by qPCR analyses. (C) and (D) Dual luciferase reporter assay revealed that miR-328-3p significantly inhibited SLC7A5 and SLC2A1 3′ UTR-luciferase reporter activities in HEK293 cells, as compared to controls. Values are the mean ± SD (n = 5–6/group); ***P < 0.001, one-way ANOVA with Bonferroni's post-hoc test. hsa, Homo sapiens.