Figure 4.

Bioengineered miR-328-3p reduces the protein levels of GLUT1 and LAT1 in human osteosarcoma cells, and subsequently decreases mTOR and p-mTOR protein levels. Cells were treated with hBERA/miR-328, control RNA or vehicle for 48 h, and protein levels were determined by Western blot analyses with selective antibodies. β-Actin was used as a loading control. Protein levels were normalized to vehicle group for comparison. Values are the mean ± SD (n = 3/group); *P < 0.05 and ^#P < 0.05, compared to control RNA and vehicle, respectively (one-way ANOVA with Bonferroni's post-hoc test).