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Intracellular and extracellular amino acid levels are not significantly altered by miR-328-3p in human osteosarcoma cells. Intracellular (A) and extracellular (B) amino acid concentrations were quantitated with an accurate and selective LC–MS/MS method at 48 h post-treatment of MG63 and 143B cells with hBERA/miR-328, control RNA and vehicle. Amino acid levels in cell lysates were normalized to corresponding protein concentrations. Values are the mean ± SD (n = 3/group).
