Fig. 1.

Experimental design. Subjects entered the scanner after a 45 min acclimatization period outside the scanner. The whole protocol consists of a series of scans of which we used the structural scan and the three resting-state scans for our present study. We selected the first 8:30 min of the third resting-state scan in order to match for length with the other resting-state scans. Stress induction took place with a highly aversive movie with a self-referential instruction. The neutral movie served as a control condition. Salivary cortisol sampling took place 20 min before scanning and ±26 min after the start of the aversive movie. Subjective stress levels were assessed at several moments during scanning with an 11-point rating scale. Heart rate was measured continuously during scanning. Other scans of our protocol that we do not use for our present study are the Hariri (an emotional face matching task), the fieldmap and a diffusion tensor imaging (DTI) scan.