Figure S1.

Effect of the 3ʹ-UTR on PXR expression in Huh-7 cells. Huh-7 cells were transiently transfected with either a pSG5-PXR or pSG5-PXR-UTR construct (200 ng) and cotransfected with pSG5-RXRα (200 ng). The next day, cells were cultured in fresh medium for 24 h (A) or for 48 h (B, C), respectively. After treatment, (A, C) total RNA was isolated and analyzed for basal levels of NR1I2 mRNA by RT-qPCR. GAPDH reference gene-normalized data are shown as the mean ± SD (n = 3) and are expressed as relative change compared to control pSG5-PXR-transfected cells (100%). (B) Samples were analyzed for expression of PXR protein by Western blotting. β-Actin was used as a loading control. Representative Western blotting results are shown. *P < 0.05, **P < 0.01 indicate a statistically significant effect of the 3ʹ-UTR region of NR1I2 mRNA.