Figure S3.

Dexamethasone stabilizes the 3′-UTR of NR1I2 mRNA RNA in HepG2 cells. HepG2 cells were transiently transfected with pmiRGLO or pmiRGLO-UTR vector (50 ng) together with pSG5-GRα expression vector (150 ng). The next day, cells were treated with dexamethasone (DEX, 100 nmol/L), a combination of DEX (100 nmol/L)/RU486 (10 μmol/L) or vehicle (DMSO; 0.2%) for 24 h (A) or 48 h (B), respectively. Next, samples were analyzed by Dual-Luciferase Reporter Assay. Firefly luciferase activities were normalized to Renilla activities and were further normalized to the corresponding activity of the pmiRGLO vector. The data are shown as the mean ± SD (n = 3) and are expressed as fold change compared to DMSO-treated controls (defined as 1). *P < 0.05, **P < 0.01 indicates a statistically significant effect compared to vehicle-treated or DEX-treated samples.