Figure 2.

PCN-mediated activation of mouse PXR affects Nr1i2 expression in mouse liver. Mouse PXR (A) or CYP3A11 (B) mRNA expression in mice that were administered a single i.p. injection of vehicle or PCN (50 mg/kg) and sacrificed after 3, 6, 24, 48, 72, or 168 h postapplication. In addition, liver sample homogenates were analyzed for mouse PXR (C) and CYP3A11 (D) protein expression employing Western blotting. Gapdh was used as a reference gene for RT-qPCR expression data normalization, and β-actin was used for Western blotting data normalization. The data are shown as the mean ± SD (n = 5 per group) and are expressed as change relative to vehicle-treated control samples defined as 100%. Mann–Whitney test was used to compare unpaired data in mice. *P < 0.05, ^**P < 0.01, and ^***P < 0.001 indicate a statistically significant effect of PCN treatment.