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. 2019 Nov 28;10(1):171–185. doi: 10.1016/j.apsb.2019.10.009

Figure 4.

Figure 4

Effects of parental HFD on the hepatic expression of NRs in offspring. The offspring derived from parental mice fed with a LFD or HFD for 8 weeks were sacrificed at different ages after birth (n = 3–6 per group). The liver tissues were collected to determine the mRNA expression levels of NRs. The mRNA expression levels were measured using the RT-qPCR method and calculated using the 2−ΔΔCt method with normalizations to Gapdh. Data are depicted as mean ± SD. Each dot represents a fold change in gene expression of one mouse. Statistical significance between two groups was determined by an unpaired Student's t-test. *P < 0.05, **P < 0.01, ***P < 0.001, HFD versus the age-matched LFD groups.