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. 2019 Nov 28;10(1):171–185. doi: 10.1016/j.apsb.2019.10.009

Figure 7.

Figure 7

Effects of HFD-feeding durations of parental mice on the hepatic expression of NRs in offspring. After being fed with either a LFD or HFD for 4 or 18 weeks, mice were mated. The offspring were sacrificed at different ages after birth (n = 3–6 per group). The liver tissues were collected to determine the mRNA expression levels of NRs. (A)–(E) The mRNA expression of NRs in the liver of offspring derived from LFD- or HFD-fed parental mice for 4 weeks. (F) The mRNA expression levels of NRs in the liver of offspring derived from LFD- or HFD-fed parental mice for 18 weeks. The mRNA expression levels were measured using the RT-qPCR method and calculated using the 2−ΔΔCt method with normalizations to Gapdh. Data are depicted as mean ± SD. Each dot represents a fold change in gene expression of one mouse. Statistical significance between two groups was determined by an unpaired Student's t-test. *P < 0.05, **P < 0.01, ***P < 0.001, HFD versus the age-matched LFD groups.