Figure 6.
Linsitinib blocked IGF-1-stimulated OAT3 transport activity. OAT3-expressing cells were treated with IGF-1 (100 nmol/L) and with various concentrations of linsitinib for 4 h. 4-min uptake of [3H]-estrone sulfate (ES, 300 nmol/L) was then measured. Transport activity was expressed as percentage of the uptake in control cells. The data corresponded to the uptake of OAT3-expressing cells minus uptake of untreated parental cells. Values are mean ± SD (n = 3); *P < 0.05. Statistical analysis was performed using one-way ANOVA by using GraphPad Prism software (GraphPad Software Inc., San Diego, CA, USA).