Figure 2.

Immunoreactivity of protein extracts from chicken breast samples collected from broiler chickens fed a high-oleic peanut diet. Total protein extracts from chicken breast samples (eight samples per treatment) were ran using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with 75 μg of total protein per lane. Resolved proteins were transferred to a nitrocellulose membrane and immunoblotted with rabbit IgG antipeanut agglutinin antibodies (1:1000); detection was determined using chromogenic peroxidase substrate with HRP. Positive control = peanut flour extracted proteins (75 μg).