Table 2.
Emax and pEC50 values for GPCR cAMP RCP knockdown assays.
| GPCR | Peptide | Scrambled siRNA Emax |
siRNA RCP Emax |
Scrambled siRNA pEC50 |
siRNA RCP pEC50 |
|---|---|---|---|---|---|
| CLR + RAMP1 | CGRP | 100.00 (±4.33) | 73.33 (±2.88)⁎⁎⁎ | 8.97 (±0.13) | 8.76 (±0.13) |
| CLR + RAMP2 | AM | 100.00 (±4.95) | 105.70 (±4.69) | 8.90 (±0.15) | 8.72 (±0.14) |
| CRF1aR | CRF | 100.00 (±3.89) | 72.47 (±7.09)** | 9.16 (±0.12) | 9.03 (±0.14) |
| CTR | CT | 100.00 (±6.04) | 72.47 (±7.09)* | 9.09 (±0.25) | 8.87 (±0.26) |
| GIPR | GIP 1–42 | 99.99 (±17.43) | 108.60 (±13.53) | 9.07 (±0.55) | 9.32 (±0.42) |
| GLP1R | GLP1 7–36 | 100.00 (±8.66) | 98.53 (±8.32) | 10.43 (±0.33) | 10.41 (±0.33) |
| GLP2R | GLP2 1–33 | 100.00 (±6.04) | 66.16 (±4.13)* | 9.043 (±0.17) | 8.93 (±0.19) |
All values are n > 3, ±S.E.M. *, **, ****, P < .05, 0.01, 0.001, Student's t. Values are normalised to cAMP seen with 10 μM forskolin.