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. 2020 Jan 22;88(2):e00759-19. doi: 10.1128/IAI.00759-19

FIG 1.

FIG 1

Structural diagrams of pSIP409-pgsA′-HA and pSIP409-aCD11c-pgsA′-HA plasmids and detection of HA and aCD11c expression. (a) pSIP409-pgsA′-HA and pSIP409-aCD11c-pgsA′-HA were constructed as described in the text. pgsA′, anchoring sequence; HA, heterologous protein (hemagglutinin); aCD11c, scFv-CD11c. The expression of HA (b) and aCD11c (c) in L. plantarum was measured by Western blotting. HA and His were used as primary antibodies in panels b and c, respectively. M, prestained marker; lane 1, 409p′ strain; lane 2, aCD11c-HA strain; lane 3, HA strain.