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. 2019 Oct 23;71(3):1029–1038. doi: 10.1093/jxb/erz464

Fig. 1.

Fig. 1.

T-DNA insertional mutagenesis decreases SIG1 transcript and protein levels. (A) SIG1 transcript abundance in sig1-1 and sig1-2 mutants as quantified by qRT-PCR. The log2 fold change after normalization with the wild type is shown. Error bars represent ±SE of the mean of four biological replicates. (B) SIG1 protein level as estimated by immunoblotting. Representative SIG1 and actin blots are shown with the corresponding stained PVDF membrane. Both SIG1 and actin are detected on the same membrane. Numbers below each lane denote the ratio of SIG1 to actin band intensity. The percentage decreases in SIG1 relative to the wild-type control are also given. The full uncropped versions of SIG1 and actin immunoblots are given in Supplementary Fig. S5. (C) An immunoblot of SIG1 with serial dilutions of the wild-type sample. The corresponding stained membrane is also shown. Molecular weight markers are indicated on the left. The mature SIG1 protein has a predicted mol. wt of 54 kDa. The SIG1 protein, however, runs on an 11.5% (w/v) SDS–6 M urea–PAGE gel with an apparent mol. wt of ~49 kDa.