Fig. 1.

Root phenotyping platform. a 10 seeds per genotype rolled into germination paper. b Plants germinate in growth chamber and removed at 5 days. c Two representative seedlings are selected from each roll for transplantation onto labeled moist blue germination paper. d Single, transplanted seedlings are covered with moist brown germination paper and sandwiched together creating one experimental unit. e Experimental units are bound with binder clips, each placed between the metal rungs of a growth chamber with the bottom 2.5 cm submerged in water. f At 6 days, 9 days and 12 days, experimental units are individually removed, split, imaged, automatically rotated, renamed via an image processing algorithm and saved to the server database, and replaced into the growth chamber