Fig. 3.

13-MB reduces H₂O₂-induced ROS generation in HUVECs. a HUVECs were exposed to 100 µM H₂O₂, then labeled with 2.5 µM DCFH-DA for 20 min, the fluorescence intensity of ROS was detected at 1 h, 2 h, 3 h. b Cells were pretreated with 1 µM 13-MB for 24 h followed by 100 µM H₂O₂ for 2 h, labeled with 2.5 µM DCFH-DA for 20 min. The levels of ROS were detected by the use of a microplate reader. c The parallel-treated cells were analyzed by flow cytometry. ****P < 0.001