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. 2020 Jan 23;18:34. doi: 10.1186/s12967-020-02218-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 11 — Effect of adjuvants on ex vivo differentiation of naïve CD4⁺ T cells from cancer patients and healthy subjects. Isolated PBMCs were incubated with adjuvants and phenotype of CD4⁺ T cells was assessed by flow cytometry. Naïve (TN) CD4⁺ T were identified as CD4⁺/CD45RA⁺/CD62L⁺; Effector cells (TE) as CD4⁺/CD45RA⁺/CD62L⁻; Central Memory (TCM) as CD4⁺/CD45RO⁺/CD62L⁺; Effector Memory (TEM) as CD4⁺/CD45RO⁺/CD62L⁻. In the panel of Naïve cells, the samples treated with RNAdjuvant are significantly lower compared to samples treated with PBS in Healthy, OT-pre and OT-post subjects (p < 0.01)