Fig. 3.

CircPTK2 promoted aggressive phenotypes of CRC cells in vitro. Cells were transfected with circPTK2 siRNA or circPTK2- overexpressing plasmid for 48 h. Then cells were harvested for annexin-V and PI staining. Cell apoptosis was detected with flow cytometry analysis. The cells were stained by crystal violet to evaluate cell proliferation, migration, and invasion capability. a, b The effect of circPTK2 knockdown on SW620 apoptosis. c, d The effect of circPTK2 overexpression on SW480 apoptosis. e-h Representative images and quantification results of the colony formation of cells. i-l Representative images and quantification results of the migration and invasion of cells. The data are the mean ± SEM. *P<0.05, **P<0.01, ***P<0.001. P values were calculated by one-way ANOVA