Fig. 4.

CircPTK2 promoted tumor growth and metastasis in a xenograft mouse model. SW620 or SW480 cells stably expressing luciferase were transfected with the indicated siRNAs or the indicated overexpressing-plasmid. After 72 h, cells were injected into the tail veins of nude mice (n = 6). a Representative bioluminescence images of mice bearing SW620 cells were obtained. b Luciferase activity of tumor, liver and lung was quantified in mice bearing SW620 cells. c SW620 cells, transfected with the indicated siRNAs, were injected to spleen of mice. After 21 and 42 days, livers were obtained. Representative images of liver and its HE staining were shown. d SW620 cells were injected into the tail veins of nude mice. After 7 and 14 days, lungs were obtained. Representative images of lung and its HE staining are shown. e Representative bioluminescence images of mice bearing SW480 cells were obtained. f Luciferase activity of tumor, liver and lung was quantified in mice bearing SW480 cells. g SW480 cells, transfected with the indicated overexpressing-plasmid, were injected to spleen of mice. After 21 and 42 days, livers were obtained. Representative images of liver and its HE staining are shown. h SW480 cells were injected into the tail veins of nude mice. After 7 and 14 days, lungs were obtained. Representative images of lung and its HE staining are shown. The data are the mean ± SEM. *P<0.05, **P<0.01, ***P<0.001. P values were calculated by one-way ANOVA