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. 2020 Jan 23;18:36. doi: 10.1186/s12967-020-02221-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effect of IL-24 treatment on the viability of H₂O₂ treated FHs74Int cells. The effect of IL-24 on the viability of the H₂O₂ treated cells was investigated by MTT (a), LDH (b) and Annexin V apoptosis (c) assays. Results of MTT and LDH assays (n = 5) are presented as the percentage of the untreated group (0 µM H₂O₂ control), and as the percentage of total cells in the case of Annexin V apoptosis assay (n = 3 × 3 replicates). The mRNA expression of IL1A (d), IL1B (e), IL6 (f) and TNF (g) was determined by real-time RT-PCR in comparison with RPLP0 as internal control (n = 5). Results are presented as mean + SD. *p < 0.05 vs. 0 µM H₂O₂control (two-way ANOVA); ^#p < 0.05 vs. IL-24 +0 µM H₂O₂ (two-way ANOVA); §p < 0.05 control vs. IL-24 at the concerning H₂O₂ concentration (multiple t-test); $p < 0.05 vs. control (two-way ANOVA); ^€p < 0.05 vs. H₂O₂ (two-way ANOVA)