Figure 3.

In vitro transfection with miR‐145 mimics or inhibitors strongly induced the proliferation, migration, and apoptosis of HASMCs. A, Proliferation curves of HASMCs transfected with control, miR‐145 mimics, mimics control, inhibitors, and inhibitor control. The proliferation of HASMCs was found to be significantly suppressed after 72‐h treatment with miR‐145 inhibitors. In contrast, miR‐145 mimics promoted the proliferation of HASMCs compared with miR‐145 inhibitor control (n = 5 per group). B and D, The Transwell test results showed that HASMC migration in the miR‐145 inhibitor group was stunted. However, the migration markedly increased in the miR‐145 mimics group. Data were expressed as the number of migrating cells per high‐power field (n = 5/group). C and E, transfection with miR‐145 mimics promoted the apoptosis of HASMCs, as shown by flow cytometric analysis (n = 5/group). D and E, Data are mean ± standard deviation. All assays were conducted in three sets