Table IV.
Differences between GenoType MTBDRplus line probe assay and Xpert MTB/RIF assay
| Line probe assay | Xpert MTB/RIF |
|---|---|
| Mycobacterium tuberculosis isolates or AFB-positive samples | Directly performed on clinical samples Initial test for tuberculous meningitis |
| Diagnose MDR TB | Identifies M. tuberculosis and diagnoses drug resistance |
| Detects RIF and INH resistance | Detects RIF resistance only |
| It takes two to three days for positive results | It takes three to four hours for positive results |
| DNA strip technology | Cartridge based technology |
| It is a multiplex PCR in combination with reverse hybridization-based technique | It is an RT-PCR-based assay |
| Suitable for use at national/central reference laboratories | Xpert MTB/RIF suitable for public health settings as well |
| Commercial kits INNO-LiPA RIF TB (Innogenetics, Ghent, Belgium) GenoType MTBDRplus (Hain Life-Science, Nehren, Germany) | Xpert MTB/RIF (Cepheid Sunnyvale, USA) |