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. 2020 Jan 14;3(2):e201900515. doi: 10.26508/lsa.201900515

Figure 2. Characterization of the ECM niche for TSCs in vivo.

Figure 2.

(A) Schematic views of in situ integrin ligand detection. Left, integrin αβ dimer on the cell surface; middle, αβ dimerized recombinant integrin ectodomain; right, in situ integrin ligand detection. (B) Comprehensive analyses of the ECM niche using E5.5 embryonic sections. Magenta, in situ binding of recombinant integrins; green, immunoreactivities for anti-laminin α1 (for integrins α3β1, α6β1, α6β4, α7x1β1, α7x2β1, α8β1, α10β1, α11β1, αVβ5, αVβ6, and αVβ8) and anti-laminin γ1 (for integrins α1β1, α2β1, α4β1, α5β1, α9β1, and αVβ3) mAbs; white, area double-positive for magenta and green signals; dotted lines, ExE basement membrane. The arrowheads indicate recombinant integrins bound to the ExE basement membrane. Bars, 50 μm.