Fig. 4.

Whole-mount immunostaining with the mouse anti-NF primary antibody on nerve fibers in adult mouse biceps brachii and tibialis anterior. (a), (b) 3-D reconstruction of the biceps brachii and the tibialis anterior, immunostained with the iDISCO and m-iDISCO methods, respectively (Video 1). (c), (d) The $150\text{-}\mu \mathrm{m}$-thick MIPs of the images in $XZ$ direction. The rough locations are marked in (a) and (b) with translucent red frames, and the green arrows indicate the labeled nerve fibers. (e), (f) Normalized intensity profiles of the background signal at the surface and the inside of the muscles. The locations are marked in (c) and (d) with the dashed lines. (Video 1, MPEG, 11.6 MB [URL: https://doi.org/10.1117/1.NPh.7.1.015003.1]).