Table 1.
Bos taurus primers used for real‐time PCR
| Gene name | Accession number | Primers sequence | Product size, bp |
|---|---|---|---|
| β‐actin | NM_173979.3 |
F: 5’‐CTCTTCCAGCCTTCCTTCCT−3’ R:5’‐GCAGTGATCTCTTTCTGC−3’ |
178 |
| αS1‐casein | NM_181029.2 |
F: 5’‐ACTGAGGATCAAGCCATGGAAG−3’ R:5’‐GAATGTGCTTCTGCTCAACACT−3’ |
100 |
| β‐casein | XM_010806178.2 |
F: 5’‐ACCAGCCTCTTCCTCCAACT−3’ R:5’‐GCCTGAATGGGCATATCTCT−3’ |
124 |
| JAK2 | XM_005209981.4 |
F: 5’‐CAAGACCAGATGGATGCCCAG−3’ R:5’‐ACTCGAACTGCTAGGTCTCTGA−3’ |
103 |
| STAT5 | NM_174617.4 |
F: 5’‐GAGAACACCCGCAATGATTAC−3’ R:5’‐TCACCGACTCTGCTCCACG−3’ |
151 |
| ELF5 | NM_001024569.1 |
F: 5’‐CATCCGCTCACAAGGTTACTC−3’ R:5’‐CTCGCACAAATTCCCATAGAT−3’ |
170 |
| PI3K | NM_001206047.1 |
F: 5’‐GTCTGGACCTTCGGATGCTAC−3’ R:5’‐TAAACTCCTCAATGGCTCGGT−3’ |
213 |
| AKT1 | NM_173986.2 |
F: 5’‐GCACAAGCGAGGTGAGTACAT−3’ R:5’‐GCCACGGAGAAGTTGTTGAG−3’ |
138 |
| mTOR | XM_002694043.6 |
F: 5’‐CGAAGAACCAATTATACCCGC−3’ R:5’‐CATAGCAACCTCAAAGCAGTCC−3’ |
153 |
| S6K1 | NM_205816.1 |
F: 5’‐AATGCTGCTTCTCGTCTTGGA−3’ R:5’‐CAGTTCTTCCCAGTTAATATGTCT−3’ |
90 |
| eIF4E | NM_174310.3 |
F: 5’‐CCCGCCTACAGAAGAAGAGA−3’ R:5’‐CAGTATCAAACTTAGAGATCAATCG−3’ |
164 |
| ACC | NM_174224.2 |
F: 5’‐GGAGACAAACAGGGACCATTAC−3’ R:5’‐GTGGAAGGAATGCTTGGGAG−3’ |
187 |
| FASN | NM_001012669.1 |
F: 5’‐GACCTGGGAGGAGTGTAAGC−3’ R:5’‐GCGATAGCGTCCATGAAGTA−3’ |
198 |
| SCD | NM_173959.4 |
F: 5’‐CCACGTTCTTCATTGATTGC−3’ R:5’‐CAGCCACTCTTGTAGCTTTCC−3’ |
121 |
| LPL | NM_001075120.1 |
F: 5’‐TCACTTCAACCACAGCAGCA−3’ R:5’‐GATGACGTTGGAGTCCGGTT−3’ |
127 |
| PPARγ | NM_181024.2 |
F: 5’‐TTCCGTTCCCAAGAGCTGAC−3’ R:5’‐TGGGGATACAGGCTCCACTT−3’ |
98 |
| SREBP1 | NM_001113302.1 |
F: 5’‐CGCTCTTCCATCAATGACA−3’ R:5’‐TTCAGCGATTTGCTTTTGTG−3’ |
188 |