TABLE 1.
DNA concentration, yield and purity of urinary and salivary DNA
| Population |
Urine |
Saliva |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
Nanodrop |
Qubit |
Nanodrop |
Qubit |
|||||||
| DNA conc (ng/µl) | Total yield (ng DNA/ml biofluid) | A260/A280 | DNA conc (ng/µl) | Total yield (ng/ml biofluid) | DNA conc (ng/µl) | Total yield (ng DNA/ml biofluid) | A260/A280 | DNA conc (ng/µl) | Total yield (ng/ml biofluid) | |
| Girls (n = 8) | 37.6 [34.3–40.5] | 626 [571–675] | 1.57 ± 0.22a | 0.43 [0.26–0.58]b | 7.20 [4.33–9.66]b | 290 [284–502] | 22,100 [18,800–41,700] | 1.87 ± 0.04a | 111 [85–160] | 9690 [6700–12,500] |
| Boys (n = 12) | 34.5 [30.4–38.2] | 575 [506–637] | 1.62 ± 0.08c | 0.11 [0.10–0.13]b | 1.83 [1.60–2.16]b | 376 [264–502] | 33,500 [25,900–38,300] | 1.85 ± 0.05c | 123 [110–176] | 12,100 [8700–15,900] |
Measurements were performed with Nanodrop and Qubit. Values are represented as median (interquartile range) for concentration and yield and mean ± sd for the A260/A280 ratio.
a
Significant differences (P < 0.001) were observed for the mean ratio of A260/A280 between urine and saliva for girls when using the Wilcoxon signed rank-sum test.
b
When using the Wilcoxon rank-sum test, significant differences (P < 0.001) of urinary yield and concentration were observed between boys and girls, when measured by Qubit.
c
Significant differences (P < 0.001) were also observed for the mean ratio of A260/A280 between urine and saliva for boys when using the Wilcoxon signed rank-sum test.