TABLE 2.
Genotyping results for the CC16 SNP A38G and the UGRP1 SNP-112G/A
| Characteristic |
CC16 SNP A38G |
UGRP1 SNP-112G/A |
||
|---|---|---|---|---|
| Saliva | Urine | Saliva | Urine | |
| Total number of samples | 20 | 20 | 20 | 20 |
| Homozygous wild type (%) | 9 (45%) | 8 | 14 (70%) | 9 |
| Heterozygous (%) | 11 (55%) | 7 | 6 (30%) | 3 |
| Homozygous mutant (%) | 0 (0) | 0 | 0 (0) | 0 |
| Determined (%) | ||||
| 50 ng DNA input | 20 (100%)a | 15 (75%) | 20 (100%)a | 12 (60%) |
| 200 ng DNA input (b) | 19 (95%) | 18 (90%) | ||
Both genotyping assays were performed twice with the DNA extracted from saliva (n = 20) and urine (n = 20) 0.50 ng of DNA input (based on Nanodrop) was used.
All salivary samples yielded a 100% GCR, including for the samples A, B, C, D, E, F, randomly selected for the DNA integrity verificatio, [with C not impacted by the lower DIN value (see Fig. 1)]. This is in contrast with the selection of the 6 urinary DNA samples for which samples C and F resulted in undetermined CC16 SNP genotype and sample F in undetermined UGRP1 SNP genotype.
The failed urinary samples were repeated with 200 ng DNA input. As all saliva samples could be successfully genotyped, the percentage of each obtained genotype based on the total amount of samples tested, was calculated based on the saliva values (indicated between brackets).