Figure 1.

TRAF6 expression is upregulated in renal tissues in LPS mice. (A) Western blot analysis and densitometric analysis of TRAF6 levels in renal tissues. (B) Immunofluorescence staining for TRAF6 (red) and AQO-1 (green) showing immunoreactivity for TRAF6 in tubular epithelial cells [scale bar=50 μm]. Merged images (yellow) showing colocalization. (C) Immunofluorescence staining of kidney tissues for TRAF6 (red) and WT-1 (green) [scale bar=50 μm]. WT-1 was used as a marker of podocytes (NC=negative control; n=7 per group; * vs. the NC group; *** P<0.001).