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. 2020 Jan 13;26:e919698-1–e919698-12. doi: 10.12659/MSM.919698

Figure 2.

Figure 2

Knocking down TRAF6 leads to the preservation of renal function in LPS-treated mice and the normalization of tissue apoptosis. (A) The efficiency of TRAF6 knockdown following AAV2-shTRAF6 administration. (B, C) The levels of serum urea nitrogen (UN) and creatinine (Cr) in LPS-treated mice and mice treated with AZD. (D) Representative images of H&E staining [scale bars= 100 μm (upper) and 50 μm (below)]. (E, F) The mRNA levels of TGF-β, Col-IV and Bax and Bcl-2 in the kidney tissues of mice, as determined by qPCR [the mRNA levels were normalized to β-actin]. (G) Apoptotic DNA fragmentation in the renal tissues of LPS mice, as detected by TUNEL staining (green). The tissues were counterstained with DAPI (blue) [scale bar= 50 μm. (n=7 per group; * vs. the NC group; # vs. the NC+LPS group; # P<0.05, ** and ## P<0.01, *** P<0.001).