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. 2020 Jan 13;26:e919698-1–e919698-12. doi: 10.12659/MSM.919698

Figure 3.

Figure 3

Knockdown of TRAF6 normalizes LPS-induced inflammatory factor release and oxidative stress in vivo. (A, B) The release of the inflammatory factors TNF-α and IL-6 in the serum, as detected by ELISA. (C, D) The protein levels of TNF-α and IL-6 in renal tissues, as detected by ELISA. (E) Representative immunohistochemcal staining of renal tissues for macrophage marker CD68. (E–G) LPS injection increased ROS production in the renal tissues of mice, as shown by increased 3-nitrotyrosine (3-NT; brown in E) chromogen staining and DHE (red in F) immunofluorescence. AAV2-shTRAF6 administration in mice prevented LPS-induced oxidative stress, as evidenced by reduced 3-NT staining and DHE fluorescence. (H, I) Antioxidant responses were measured in renal tissues by assessment the mRNA levels of Nrf2 and HO-1 (n=7 per group; * vs. the NC group; # vs. the NC+LPS group; ## P<0.01, *** and ### P<0.001).