Table 1. Taqman probe detection of linkage between SNP mutations and the mutant phenotype.
Genotype (Fluorescent signal) | Pwt: 8 | Pm: 8 | F2m: 625 | F2wt: 63 |
---|---|---|---|---|
AATG (High peak individuals) | 0 | 8 | 625 | 0 |
GACT/AATG (Medium-high peak individuals) | 0 | 0 | 0 | 35 |
GACT (Undetected individuals) | 8 | 0 | 0 | 28 |
Pwt means parent individuals with wild-type character, Pm indicates parent individuals with mutant phenotype, F2m indicates number of individuals with mutant phenotype in F2 population, F2wt indicates individual number of F2 population with wild-type character.