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. 2020 Jan 10;16(1):e1008268. doi: 10.1371/journal.ppat.1008268

Fig 2. Validation of selected hits by using shRNA-mediated knockdown in latently infected BCBL1 cells.

Fig 2

(A) RT-qPCR analysis of the expression of epigenetic factors in BCBL1 cells 72 hours after shRNA lentiviral infection. The percentage of remaining transcripts is shown relative to the non-targeting control shRNA (shControl). (B) Immunoblot analysis to confirm the shRNA knockdown of MBD3, GATAD2B, and KDM2B at the protein level. (C) Effects of the shRNA inhibition of host epigenetic factors on viral gene expression in BCBL1 cells. RT-qPCR was performed for 29 viral genes including latent, immediate early (IE), early (E), and late (L) genes. (D) Immunoblot analysis of viral proteins following the shRNA depletion of the host epigenetic factors. Tubulin was used as a loading control.