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. 2020 Jan 10;16(1):e1008268. doi: 10.1371/journal.ppat.1008268

Fig 7. Effect of KDM2B depletion on viral gene expression in different epithelial cell types during de novo KSHV infection.

Fig 7

(A) Knockdown efficiency of KDM2B by shRNA lentivirus infection in primary human gingival epithelial (HGEP) cells. HGEP cells were infected with shControl or shKDM2B lentiviruses for 2 days, followed by KSHV infection. RT-qPCR was performed 6 and 48 hours after KSHV infection to measure viral gene expression. (B and C) Viral DNA load in KSHV-infected HGEP cells at 6 hpi and 48 hpi. (D) RT-qPCR analysis of viral gene expression. (E) RT-qPCR analysis of viral gene expression in shControl and shKDM2B SLK cells 24 and 72 hours after KSHV infection. Note for panel A-E: significance test was performed between shControl and shKDM2B samples (*p<0.05). NS, not significant. (F) Immunoblots showing viral ORF6 protein expression at 24 hpi and 72 hpi.