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. 2020 Jan 14;9:e50356. doi: 10.7554/eLife.50356

Key resources table.

Reagent type
(species) or resource
Designation Source or reference Identifiers Additional
information
Strain, strain background (Mus musculus, female) C57Bl/6J Charles Rivers, Lyon, France C57Bl/6J Female
Chemical compound, drug THC
(Tetrahydrocannabinol)
THC-Pharm-GmbH Natural THC
Chemical compound, drug Cremophor EL Sigma-Aldrich C5135; Kolliphor EL
Chemical compound, drug 0.9%, NaCl physiological saline Laboratorios Ern Vitulia
Chemical compound, drug Ethanol Scharlab ET00051000
Chemical compound, drug Vaporised Isoflurane in oxygen Virbac Vetflurane 4% V/V for induction; 2.5% V/V for maintenance
Chemical compound, drug Optimal cutting temperature compound Sakura finetek 25608–930 Item code 4583
Biological sample (Equus asinus) Normal donkey serum Sigma-Aldrich D9663-10ML 3% in PBS with 0.3% Triton X-100
Biological sample (Capra aegagrus hircus) Normal goat serum Vector lab S-1000
Antibody Rabbit polyclonal anti-beta-III tubulin antibody Abcam ab18207 (1:2000)
Antibody Donkey polyuclonal anti-rabbit Alexa Fluor A488 antibody Thermo Fisher Scientific A21206 (1:1000)
Antibody Goat polyclonal anti-rabbit Alexa Fluor A555 antibody Abcam ab150078 (1:1000)
Chemical compound, drug DAPI Fluoromount-G mounting media SouthernBiotech 0100–20
Chemical compound, drug Calcium EDTA (Sodium calcium edetate) Sigma-Aldrich Sodium calcium edetate
Commercial assay or kit Enzyme-linked immunosorbent assay; ELISA Calbiotech ES180S-100
Software, algorithm NIH Image J software Wayne Rasband
Software, algorithm GraphPad Prism 8 GraphPad Software, Inc
Software, algorithm IBM SPSS 23 software IBM Corporation
Software, algorithm Smart 3.0 videotracking software Panlab