Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Mus musculus, female) | C57Bl/6J | Charles Rivers, Lyon, France | C57Bl/6J | Female |
Chemical compound, drug | THC (Tetrahydrocannabinol) |
THC-Pharm-GmbH | Natural THC | |
Chemical compound, drug | Cremophor EL | Sigma-Aldrich | C5135; Kolliphor EL | |
Chemical compound, drug | 0.9%, NaCl physiological saline | Laboratorios Ern | Vitulia | |
Chemical compound, drug | Ethanol | Scharlab | ET00051000 | |
Chemical compound, drug | Vaporised Isoflurane in oxygen | Virbac | Vetflurane | 4% V/V for induction; 2.5% V/V for maintenance |
Chemical compound, drug | Optimal cutting temperature compound | Sakura finetek | 25608–930 | Item code 4583 |
Biological sample (Equus asinus) | Normal donkey serum | Sigma-Aldrich | D9663-10ML | 3% in PBS with 0.3% Triton X-100 |
Biological sample (Capra aegagrus hircus) | Normal goat serum | Vector lab | S-1000 | |
Antibody | Rabbit polyclonal anti-beta-III tubulin antibody | Abcam | ab18207 | (1:2000) |
Antibody | Donkey polyuclonal anti-rabbit Alexa Fluor A488 antibody | Thermo Fisher Scientific | A21206 | (1:1000) |
Antibody | Goat polyclonal anti-rabbit Alexa Fluor A555 antibody | Abcam | ab150078 | (1:1000) |
Chemical compound, drug | DAPI Fluoromount-G mounting media | SouthernBiotech | 0100–20 | |
Chemical compound, drug | Calcium EDTA (Sodium calcium edetate) | Sigma-Aldrich | Sodium calcium edetate | |
Commercial assay or kit | Enzyme-linked immunosorbent assay; ELISA | Calbiotech | ES180S-100 | |
Software, algorithm | NIH Image J software | Wayne Rasband | ||
Software, algorithm | GraphPad Prism 8 | GraphPad Software, Inc | ||
Software, algorithm | IBM SPSS 23 software | IBM Corporation | ||
Software, algorithm | Smart 3.0 videotracking software | Panlab |