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. 2020 Jan 8;9:e50109. doi: 10.7554/eLife.50109

Figure 5. Histone modifications in SPT deletion and degron strains.

(A, B) Quantitation of total H3K18-Ac and H2B-Ub in the SPT deletion and degron strains, normalized to the TFIIH subunit Tfg2 as a loading control. Data are from Figure 5—figure supplement 1. In panel (B), time after IAA or DMSO addition is indicated. (C) Box plot showing the ChIP-seq assayed log2 change per gene in H3K18-Ac signal in the SAGA deletion mutants and Spt3/7 degron strain. Degron strain was treated for 30 min with IAA before formaldehyde crosslinking. Mean values from replicate experiments are plotted.

Figure 5—source data 1. Quantification of western blot results presented in Figure 5—figure supplement 1.
Data was used to make graphs in Figure 5AB.

Figure 5.

Figure 5—figure supplement 1. Histone modifications in SPT deletion and degron strains.

Figure 5—figure supplement 1.

(A, B) Quantitation of total H3K18-Ac and H2B-Ub in the SPT deletion and degron strains as indicated. Depletion of the degron-tagged subunits is also shown. Data used for quantitation in Figure 5. Variable amounts of wild-type samples without IAA treatment were loaded to allow quantitation of histone modifications. Blots were probed with α-H3K18-Ac, α-H2B-Ub, α-Tfg2, α-H3 polyclonal antibodies and α-V5 monoclonal antibodies (for the degron-tagged subunit) as indicated.
Figure 5—figure supplement 2. ChIP-seq analysis of H3K18-Ac signal in the SAGA deletion mutants and Spt3/7 degron strains.

Figure 5—figure supplement 2.

The degron strain was treated for 30 min with IAA or DMSO before formaldehyde crosslinking. (A) Genome browser images showing comparison of normalized signal at representative genomic locations. Mean values from replicate experiments are shown. (B) Box plot showing the log2 change per gene in H3K18-Ac signal in the SAGA deletion mutants and Spt3/7 degron strain. Genes are divided into two classes according to results of the k-means clustering analysis of SAGA deletion and TFIID degron experiments from Figure 1. Mean values from replicate experiments are plotted. (C) Correlation matrix of Pearson correlation coefficient values between log2 changes in H3K18-Ac signal in the SAGA deletion mutants and Spt3/7 degron strain. Mean values from replicate experiments were used.