Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Schizosaccharomyces pombe) | Wild-type | S Forsburg | FY527 | ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | Fim1p-SNAP | This study | JB135 | fim1-SNAP-kanMX6 ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | Acp2p-mEGFP/Fim1p-SNAP | This study | JB150 | acp2-mEGFP-kanMX6 fim1-SNAP-kanMX6 ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | Pil1p-SNAP | (Lacy et al., 2017) | JB198 | pil1-SNAP-kanMX6 ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | Clc1p-SNAP | This study | JB202 | clc1-SNAP-kanMX6 ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | SNAP-Act1p | This study | JB216 | 41nmt1-SNAP-actin-leu+ ade6-M216 his3-D1 ura4-D19 h- |
| Strain, strain background (S. pombe) | SNAP-Myo1p | This study | JB304 | SNAP-myo1 fex1Δ fex2Δ ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | Acp1p-SNAP | This study | JB305 | acp1-SNAP fex1Δ fex2Δ ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | End4p-SNAP | This study | JB307 | end4-SNAP fex1Δ fex2Δ ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | Arc5p-SNAP | This study | JB346 | arc5-SNAP fex1Δ fex2Δ ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | Acp1p-mEGFP | This study | JB366 | acp1-mEGFP fex1Δ fex2Δ ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | SNAP-Wsp1p | This study | JB393 | SNAP-wsp1 fex1Δ fex2Δ ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | mEGFP-Wsp1p | This study | JB385 | mEGFP-wsp1 fex1Δ fex2Δ ade6-M216 his3-D1 leu1-32 ura4-D18 h- |
| Strain, strain background (S. pombe) | Acp1p-mEGFP/Fim1p-mCherry | (Berro and Pollard, 2014b) | JB54 | acp1-mEGFP-kanMX6 fim1-mCherry-NatMX6 ade6-M216 his3-D1 leu1-32 ura4-D18 h+ |
| Strain, strain background (S. pombe) | Fim1p-mEGFP | (Berro and Pollard, 2014b) | JB57 | fim1-mEGFP-NatMX6 his3-D1 leu1-32 ura4-D18 leu1-32 h+ |
| Strain, strain background (S. pombe) | mEGFP-Myo1p | (Sirotkin et al., 2005) | TP195 (JB205) | kanMX6-Pmyo1-mGFP-myo1 leu1-32 ura4-D18 his3-D1 ade6 h- |
| Other | SNAP-Cell 647-SiR | New England Biolabs | S9102S | Dissolved to 100 µM in DMSO and aliquoted, stored −80°C |
| Software, algorithm | Matlab | Mathworks | Scripts are included as Source code | |
| Software, algorithm | FIJI ImageJ | (Schindelin et al., 2012; Schneider et al., 2012) | ||
| Software, algorithm | Python Microscopy Environment (PYME) | www.python-microscopy.org; (Baddeley et al., 2011) |