Table 2. Statistics for single-molecule tracks of SNAP-tag fusion proteins.
| Name | Lifetime Median, sec | Lifetime Mean, sec | Lifetime S.D., sec* | Lifetime 95%ile, sec | End-to-end distance Median, nm |
n tracks | N movies (datasets with > 40 tracks) | N samples | D, nm2/sec † |
|---|---|---|---|---|---|---|---|---|---|
| Act1p | 1.1 | 1.5 | 0.12 | 4 | 159 | 7660 | 18 (18) | 2 | 2955 |
| Acp1p | 1.1 | 1.4 | 0.19 | 3.6 | 167 | 4977 | 24 (21) | 5 | 3874 |
| Fim1p | 1 | 1.5 | 0.17 | 4.2 | 154 | 16,490 | 12 (12) | 2 | 2855 |
| Arc5p | 1.2 | 1.6 | 0.32 | 4.2 | 166 | 4576 | 17 (13) | 2 | 3337 |
| Myo1p | 1.1 | 1.6 | 0.16 | 3.9 | 156 | 3951 | 10 (10) | 2 | 1678 |
| Wsp1p | 0.9 | 1.2 | 0.13 | 2.6 | 147 | 4379 | 41 (32) | 5 | 1869 |
| Clc1p | 1.1 | 1.5 | 0.16 | 3.7 | 168 | 2718 | 19 (18) | 3 | 2770 |
| End4p | 1.1 | 1.6 | 0.24 | 4.1 | 148 | 3983 | 31 (17) | 5 | 1381 |
| Pil1p | 1.2 | 2.0 | 0.67 | 5.9 | 134 | 446 | 6 (4) | 1 | 517 |
*Standard Deviation is determined across means calculated from individual datasets (each movie), only counting movies with >40 tracks.
†Apparent diffusion coefficient calculated from mean squared-displacement over time for time windows < 1 s (10 points), as in Figure 5.