Table 3. Statistics for single-molecule tracks of SNAP-tag fusion proteins imaged under alternate conditions.
| Name | Median, sec | Mean, sec | S.D., sec * | 95%ile, sec | N tracks | N movies (datasets with > 40 tracks) |
N samples | D, nm2/sec † |
|---|---|---|---|---|---|---|---|---|
| Acp1p [high laser power] |
1.1 | 1.43 | 0.12 | 3.7 | 1968 | 11 (11) | 2 | 3367 |
| Acp1p [fixed]‡ |
12.0 | 15.3 | n.d. | 54.1 | 53 | 6 | 1 | n.d. |
* Standard Deviation is determined across means calculated from individual datasets (each movie), only counting movies with >40 tracks.
† Apparent diffusion coefficient calculated from mean squared-displacement over time for time windows < 1 s (10 points).
‡ Acp1p-SiR sample fixed with formaldehyde and imaged under the same illumination conditions (low power) and camera settings as live samples (Table 2).