Figure 3.

Increase in SC proliferation and decrease in SC apoptosis with ADSC‐Exo treatment. A, Western blot analysis showed that ADSC‐Exo expressed CD9, CD63, and CD81, but ADSCs did not express these proteins. B, The cell proliferation curve of SNI‐SCs treated with different concentrations of ADSC‐Exo. Repeated measurement variance analysis, F = 19.2, P < 0.001. LSD t test, P < 0.05. C, Apoptosis of Sham‐SCs, SNI‐SCs, and SNI‐SCs + Exo was analyzed by Annexin V and PI staining. D, The apoptosis rate of SNI‐SCs decreased significantly after treatment with ADSC‐Exo. Data represent the mean ± SD values. **P < 0.01, one‐way ANOVA. E, Immunofluorescence staining showed that the expression of MPZ was increased in SNI‐SCs treated with ADSC‐Exo. Scale bar: 20 µm