Figure 4.

GNX reduced microglial cell density and enhanced clearance of myelin debris in the vicinity of the demyelination lesion. (A) and (B) show immunofluorescent staining image of damaged myelin basic protein (dMBP; green) and the microglial marker Iba1 (red) in vehicle‐treated and GNX‐treated rats, respectively. Arrowheads indicate microglia engulfing myelin debris. C, GNX significantly reduced the density of Iba1⁺ cells in the vicinity of the demyelination lesion compared to vehicle treatment (P < .05). The fraction area covered by dMBP (D) and the average OD of dMBP (E) were significantly reduced by GNX treatment compared to vehicle animals (P < .05 and P < .01, respectively). Scale bar = 50 µm