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. 2019 Jul 22;26(2):240–250. doi: 10.1111/cns.13195

Table 1.

Primary antibodies used in immunofluorescence and Western blot

Primary antibodies Dilution Company Catalogue # Secondary antibodies
Mouse monoclonal antibody anti‐MAG 1:1000 Abcam, Cambridge, MA, USA ab89780 HRP‐conjugated donkey anti‐mouse IgG
Mouse monoclonal antibody anti‐MOG 1:1000 Millipore, MA, USA MAB5680 HRP‐conjugated donkey anti‐mouse IgG
Mouse monoclonal antibody anti‐CNPase 1:2000 Sigma‐Aldrich, St. Louis, Mo., USA C5922 HRP‐conjugated donkey anti‐mouse IgG
Mouse monoclonal antibody anti‐myelin basic protein 1:5000 Calbiochem, Billerica, MA, USA NE1019 HRP‐conjugated donkey anti‐mouse IgG
Rabbit polyclonal antibody anti‐actin 1:5000 Sigma‐Aldrich, St. Louis, Mo., USA A2066 HRP‐conjugated donkey anti‐rabbit IgG
Mouse monoclonal antibody anti‐iNOS 1:1000 BD Biosciences, San Jose, CA, USA 610432 HRP‐conjugated donkey anti‐mouse IgG
Mouse monoclonal antibody anti‐arginase‐1 1:1000 BD Biosciences, San Jose, CA, USA 610708 HRP‐conjugated donkey anti‐mouse IgG
Rabbit polyclonal antibody anti‐damaged myelin basic protein 1:1000 Millipore, MA, USA AB5864 Alexa Fluor 488 donkey anti‐rabbit IgG
Goat polyclonal antibody anti‐Iba1 1:2000 Abcam, Cambridge, MA, USA ab107159 Alexa Fluor 555 donkey anti‐goat IgG
Rabbit polyclonal antibody anti‐GFAP 1:5000 Sigma‐Aldrich, St. Louis, Mo., USA G9269 Alexa Fluor 555 donkey anti‐rabbit IgG
Goat polyclonal antibody anti‐neurofilament 1:2000 Santa Cruz Biotechnology, Santa Cruz, CA, USA sc‐16143 Alexa Fluor 488 donkey anti‐goat IgG
Mouse monoclonal antibody anti‐myelin basic protein 1:2000 Calbiochem, Billerica, MA, USA NE1019 Alexa Fluor 555 donkey anti‐mouse IgG
Mouse monoclonal antibody anti‐GABAARγ2 1:200 Millipore, MA, USA MABN875 Alexa Fluor 488 donkey anti‐mouse IgG