Figure 6.

Effects of treatment with Met or RAP on PC12 cell apoptosis induced by high CORT. PC12 cells were exposed to CORT (10 μmol/L) in the presence of Met (2 mmol/L) or RAP (200 nmol/L). After treatment for 24 h, cells were harvested, and then, cell apoptotic rates of PC12 cells were measured by flow cytometry. A, Representative images of cell apoptotic rates determined by flow cytometry with annexin V/PI double staining. B, Quantitative analyses the percentage of PC12 cell apoptosis. Data were presented as mean ± SEM. ^# P < .05 vs CORT group, **P < 0.01vs control group