Figure 7.

Effects of treatment with Met or RAP on proapoptotic protein Cyt‐c and cleaved caspase‐3 expression in PC12 cells exposed to high CORT. PC12 cells were administrated with CORT (10 μmol/L) in the presence of Met (2 mmol/L) or RAP (200 nmol/L) for 24 h. All the cells were collected, and then, proteins were extracted from cell lysates for Western blot analysis. Each lane was loaded with 20 μg of proteins for all experiments. A, Representative blots of GAPDH (36 kDa), Cyt‐c (14 kDa), and cleaved caspase‐3 (17 kDa) in PC12 cells. B, C, Quantitative analyses of the relative optical density values of Cyt‐c and cleaved caspase‐3 to GAPDH were performed by Sigma Scan Pro5 software. All the data were expressed as mean ± SEM. *P < .05 vs control group, ^#P < .05 vs CORT group