Fig. 4. Amounts of both sense and antisense transcripts depend on the sense promoter.

Measurements of the amounts of RNAs produced from bulk transcription reactions on templates with an unmodified (Fig. 1a) or a scrambled (“Methods”) λ P_R′ promoter. RNAs were reverse transcribed using sense (S) or antisense (AS) specific primers (Supplementary Fig. 5A) and the amounts of cDNA produced were measured by qPCR. Graph shows mean ± s.e.m. of three experiments. All individual measurements from these and additional control samples are shown in Supplementary Fig. 5B.