Fig. 3. EC-specific deletion of ZEB1 impairs angiogenesis-dependent bone formation.

a Representative images of freshly dissected tibia of 3-week-old Zeb1^WT and Zeb1^ΔEC mice (n = 5, each). Scale bar, 50 mm. b Representative micro-CT images of trabecular bone (top panels) and cortical bone (bottom panels) of 3-week-old Zeb1^WT and Zeb1^ΔEC mice (n = 5, each). Scale bar, 0.2 mm. c Quantification of Tb. BV/TV trabecular separation (Tb. Sp.), Tb. Nb. trabecular thickness (Tb. Th.), cortical thickness (Ct. Th.) in the tibia as shown in b (n = 5 independent experiments). d Representative images of calcein double labeling of Zeb1^WT and Zeb1^ΔEC tibia (n = 5, each). Scale bar, 100 μm. e Quantification of dynamic bone formation in tibia as shown in d (n = 5 independent experiments). f, h, j Representative images of 3-week-old Zeb1^WT and Zeb1^ΔEC tibia (n = 5, each) immunostained with Runx2 (f), Osterix (h), and ALP (j). Scale bar, 100 μm. g, i, k Quantification of Runx2⁺ (g), Osterix⁺ (i), and ALP⁺ (k) cells in the tibia as shown in f, h, j (n = 5 independent experiments). l Representative images for Alizarin red S staining of 3-week-old Zeb1^WT and Zeb1^ΔEC tibia (n = 5, each). Scale bar, 100 μm. All data are represented as mean ± SD. **P < 0.01; NS not significant. Differences are tested using unpaired two-tailed Student’s t-test. The source data are provided as a Source Data file.