Fig. 8. Treatment with Zeb1-packaged cationic liposomes has therapeutic effects on OVX-induced bone loss.

a Representative micro-CT images of trabecular bone (top panels) and cortical bone (bottom panels) of the tibia in Sham and OVX mice that were i.v. injected with 4 μg Lipo.-Vector-GFP or Lipo.-ZEB1-GFP for 6 consecutive weeks (designed Sham + Lipo.-Vector-GFP, OVX + Lipo.-Vector-GFP, and OVX + ZEB1-GFP mice, respectively; n = 5, each). Scale bar, 0.2 mm. b Quantification of bone architectures in the tibia as shown in a (n = 5 independent experiments). c Runx2 and Osterix immunostaining of the tibia as described in a (n = 5, each). Scale bar, 100 μm. d Quantification of Runx2⁺ and Osterix⁺ cells in the tibia as shown in c (n = 5 independent experiments). All data are represented as mean ± SD. **P < 0.01, *P < 0.05. Differences are tested using one-way ANOVA with Tukey’s post hoc test (b, d). The source data are provided as a Source Data file.